1) Outline the principle of Electrospray Ionisation. In what situations would you use an alternative ion source
(e.g. Atmospheric Pressure Chemical Ionisation) Why is a vacuum required in mass spectrometry
2) What effect does working in positive and negative ion mode have on the m/z values Why was negative ion
mode selected
3) What effect does changing the collision energy have on the MS sensitivity What effect does too high a
collision energy have on the ions
4) What are the main advantages of LC ESI MS as a tool for identifying compounds
and also mention that from the results :
what type separation . Our sample contain 8 different ions from bileacid, The last thing is label the peaks on the first picture in the results.
Note: the first chromatogram on page 1 is the TIC (total ion current) chromatogram and all of the others are EIC
(extracted ion current) chromatograms for specific m/z species. Importantly, the last EIC chrom. on the 3rd page
is the only one you need (on that page),
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