\n1) Outline the principle of Electrospray Ionisation. In what situations would you use an alternative ion source
\n(e.g. Atmospheric Pressure Chemical Ionisation) Why is a vacuum required in mass spectrometry
\n2) What effect does working in positive and negative ion mode have on the m\/z values Why was negative ion
\nmode selected
\n3) What effect does changing the collision energy have on the MS sensitivity What effect does too high a
\ncollision energy have on the ions
\n4) What are the main advantages of LC ESI MS as a tool for identifying compounds
\nand also mention that from the results :
\nwhat type separation . Our sample contain 8 different ions from bileacid, The last thing is label the peaks on the first picture in the results.<\/p>\n
Note: the first chromatogram on page 1 is the TIC (total ion current) chromatogram and all of the others are EIC
\n(extracted ion current) chromatograms for specific m\/z species. Importantly, the last EIC chrom. on the 3rd page
\nis the only one you need (on that page),<\/p>\n","protected":false},"excerpt":{"rendered":"
1) Outline the principle of Electrospray Ionisation. In what situations would you use an alternative ion source (e.g. Atmospheric Pressure Chemical Ionisation) Why is a vacuum required in mass spectrometry 2) What effect does working in positive and negative ion Read More …<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-95552","post","type-post","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/95552","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/comments?post=95552"}],"version-history":[{"count":0,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/95552\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/media?parent=95552"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/categories?post=95552"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/tags?post=95552"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}