{"id":431401,"date":"2018-10-02T05:30:47","date_gmt":"2018-10-02T05:30:47","guid":{"rendered":"https:\/\/essaypaper.org\/what-conclusion-can-be-drawn-from-comparing-the-samples-in-b-and-d\/"},"modified":"2018-10-24T08:55:23","modified_gmt":"2018-10-24T08:55:23","slug":"what-conclusion-can-be-drawn-from-comparing-the-samples-in-b-and-d","status":"publish","type":"post","link":"https:\/\/www.benedictsol.com\/blogs\/what-conclusion-can-be-drawn-from-comparing-the-samples-in-b-and-d\/","title":{"rendered":"What conclusion can be drawn from comparing the samples in B and D?"},"content":{"rendered":"<div>\n<p><b>Enzyme Activity of an RNA Molecule<\/b><\/p>\n<p><b>Source:<\/b>\u00a0Zang, A. J., T. R. Cech. 1986. The intervening sequence RNA of\u00a0<i>Tetrahymena<\/i>\u00a0is an enzyme.\u00a0<i>Science<\/i>\u00a0231: 470\u2013475.<\/p>\n<p><b>Corresponding chapter(s) in the textbook:<\/b>\u00a0Chapter 2<\/p>\n<p><b>Review the following terms before reading the paper and working on the problem:<\/b>\u00a0synthetic oligonucleotides, 32P radioisotope, 5\u2032-end labeling, polyacrylamide gel electrophoresis, autoradiography<\/p>\n<p><b>Read the paper and answer the questions below that refers to the data described in Figure 1 of the paper.<\/b><\/p>\n<p><a rel=\"nofollow\" name=\"_GoBack\"\/><b>Be prepared to discuss the other experiments described in class.<\/b><\/p>\n<p><b>Experiment<\/b><\/p>\n<p>The function of an RNA molecule (designated L-19 IVS RNA) isolated from\u00a0<i>Tetrahymena thermophila<\/i>\u00a0(a ciliated protozoan often used as a model organism) was tested\u00a0<i>in vitro<\/i>\u00a0in this experiment. The samples in panel A did not contain L-19 IVS RNA; all other reaction mixtures contained equal amounts of L-19 IVS RNA and either 5\u2032-end labeled (with 32P) pentacytidylate (an oligoribonucleotide consisting of five C-nucleotides; panels A and B), deoxypentacytidylate (an oligodeoxyribonucleotide consisting of five C-nucleotides; panel C), or hexauridylate (an oligoribonucleotide consisting of six U-nucleotides; panel D). The samples were free of proteins. Samples were incubated for the time periods indicated in the figure, then subjected to polyacrylamide gel electrophoresis followed by autoradiography. (The direction of electrophoresis was toward the bottom for each gel.)<\/p>\n<p><b>Figure<\/b><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.homeworkmarket.com\/sites\/default\/files\/qx\/17\/08\/30\/09\/exam_1_paper_instructions_0.docx\" alt=\"\"\/><\/p>\n<p><b>Questions<\/b><\/p>\n<p>1. How were the molecules in band\u00a0<i>a<\/i>\u00a0of panel B produced?<\/p>\n<p>2. How were the molecules in band\u00a0<i>b<\/i>\u00a0of panel B produced?<\/p>\n<p>3. What conclusion can be drawn from comparing the samples in A and B?<\/p>\n<p>4. What conclusion can be drawn from comparing the samples in B and C?<\/p>\n<p>5. What conclusion can be drawn from comparing the samples in B and D?<\/p>\n<p>6. What is the function of L-19 IVS RNA? What enzyme activities are carried out by this RNA?<\/p>\n<p>\u00a9 2016 Sinauer Associates, Inc.<\/p>\n<p>\u00a0<\/p>\n<h4 style=\"text-align: center;\">&#8220;PLACE THIS ORDER OR A SIMILAR ORDER WITH ESSAY PAPER LTD AND GET AN AMAZING DISCOUNT&#8221;<\/h4>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-14111 aligncenter\" src=\"https:\/\/brainytermpapers.com\/wp-content\/uploads\/2018\/01\/get-your-custom-paper.png\" alt=\"\" width=\"380\" height=\"101\"\/><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Enzyme Activity of an RNA Molecule Source:\u00a0Zang, A. J., T. R. Cech. 1986. The intervening sequence RNA of\u00a0Tetrahymena\u00a0is an enzyme.\u00a0Science\u00a0231: 470\u2013475. Corresponding chapter(s) in the textbook:\u00a0Chapter 2 Review the following terms before reading the paper and working on the problem:\u00a0synthetic <a href=\"https:\/\/www.benedictsol.com\/blogs\/what-conclusion-can-be-drawn-from-comparing-the-samples-in-b-and-d\/\" class=\"read-more\">Read More &#8230;<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[144,16,145,146],"tags":[],"class_list":["post-431401","post","type-post","status-publish","format-standard","hentry","category-academic-papers","category-academic-writing","category-academic-writing-service","category-academy-management-assignment-help"],"_links":{"self":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/431401","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/comments?post=431401"}],"version-history":[{"count":0,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/431401\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/media?parent=431401"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/categories?post=431401"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/tags?post=431401"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}