answer this q by easy languages with following the heading<\/p>\n
no extend time my exam this morning<\/p>\n
Q1. During your practical work you cloned the Green Fluorescent Protein (GFP) into the plasmid pGLO and transformed this vector into E. Coli BL21. You induced expression of this protein based on modifications made to the vector that mimicked control of gene expression based on the Arabinose Operon control system. Describe how this system works.
Answer to include:<\/p>\n
\u2022 Overview of the Arabinose Operon and its regulation. To include control of gene expression by RNA Polymerase, structure of the Promotor, transcription initiation-elongation and termination by RNA Polymerase (10 marks)
\u2022 Description of the GFP Promotor and its regulation by ARA-C. Answer to include how this control was based on the Arabinose Operon (10 marks)<\/p>\n
\u2022 Transformation procedure and GFP induction by Arabinose by inhibiting ARA-C. (10 marks)<\/p>\n
\u2022 Class results of their transformation experiment(10 marks)<\/p>\n","protected":false},"excerpt":{"rendered":"
answer this q by easy languages with following the heading no extend time my exam this morning Q1. During your practical work you cloned the Green Fluorescent Protein (GFP) into the plasmid pGLO and transformed this vector into E. Coli Read More …<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-182631","post","type-post","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/182631","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/comments?post=182631"}],"version-history":[{"count":0,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/182631\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/media?parent=182631"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/categories?post=182631"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/tags?post=182631"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}