Transcription is nothing but the formation of RNA from DNA template using RNA polymerase.<\/p>\n
The central dogma proposed by Francis Crick in 1953 : DNA——— RNA———- Transcription Translation<\/p>\n
The formation of ss RNA molecules from ds DNA using RNA polymerase Usually 80-85% mRNA is synthesized from DNA & also there are different regions in DNA for rRNA & tRNA’s . Also the 3 different RNA’s require different RNA polymerases for synthesis. 1. RNA poly.- 1—– Synthesizes rRNA 2.RNA poly.-2—– Synthesizes mRNA 3. RNA poly.-3—– Synthesizes tRNA’s<\/p>\n
Transcription process is just similar to & has 3 phases for simplification: 1. Initiation, 2. Elongation, 3. Termination<\/p>\n
All the 3 different enzymes have similar initiation , identical elongation but different termination process. Initiation occurs when polymerase recognizes the promoter sequence. Promoter sequence is before the actual gene sequence & varies from organism to organism & also between polymerases.<\/p>\n
Once it recognizes the sequence it initiates the transcription process . In initiation process it tries to skip this DNA sequence by producing 8-9 oligonucleotide RNA abortive sequence.<\/p>\n
In elongation process , it just typifies the DNA sequence. <\/p>\n
The last step termination is done by hairpin loop or protein called rho in prokaryotes. While in eukaryotes : RNA poly 1- Terminates at specific 11bp rRNA gene sequnce. RNA poly 2- There is a sequence upstream AAUAA & downstream GU rich sequence separated by 40-60 nucleotides. There are 2 factors present on the enzyme , they recognize the sequence & cleaves the RNA now the factor meets the enzyme & terminates. RNA poly 3- Termination signal is long stretch of T bases which is recognized by poly 3 & terminates by forming dead end complex. <\/p>\n","protected":false},"excerpt":{"rendered":"
Transcription is nothing but the formation of RNA from DNA template using RNA polymerase. The central dogma proposed by Francis Crick in 1953 : DNA——— RNA———- Transcription Translation The formation of ss RNA molecules from ds DNA using RNA polymerase Read More …<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-175423","post","type-post","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/175423","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/comments?post=175423"}],"version-history":[{"count":0,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/posts\/175423\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/media?parent=175423"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/categories?post=175423"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.benedictsol.com\/blogs\/wp-json\/wp\/v2\/tags?post=175423"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}